Orhan YAPICI

Article | 2015 | Pakistan Veterinary Journal35 ( 4 )

The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37oC for 1 month. The samples were analyzed on the 0, 1st, 2nd, 3rd and 4th weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (Cmax) and area under the curve (AUC) as kinetic parameters of each s . . .ample. All of the samples were found positive both by ELISA and RT-PCR on each week. Cmax values of BVDV RNA for the storage temperatures of 4, 21 and 37°C were 356, 346 and 338 ng/μL respectively, and AUC0→4 values for the same temperatures were 1151, 1106 and 1077 week.ng/μL respectively. It was determined that storage at different temperatures for one month does not statistically influence the kinetic parameters of BVDV RNA (P>0.05). In conclusion, it can be expressed that storage of BVDV RNA at 4, 21 and 37°C for one month has no effect on the stability of BVDV RNA. - Key words: AUC, BVDV, Cmax, ELISA, RT-PC More less

Hasan GÜZELBEKTEŞ

Article | 2016 | Pakistan Veterinary Journal36 ( 4 )

Degenerative mitral valvular disease (DMVD) is the most frequent cardiac disease, causing mitral regurgitation (MR) in dogs. The purpose of this study was to compare the ratio of the regurgitant jet area (RJA) to the left atrial area (LAA) (RJA/LAA) with subtracting method to quantify regurgitant volume (RegV) and regurgitant fraction (RF) in asymptomatic Anatolian Shepherd Dogs (ASHs) with DMVD. Thirty-eight ASHs with DMVD were used as experimental group. The control group consisted of 35 healthy ASHs. In 38 ASHs with DMVD (20 B1 dogs and 18 B2 dogs), the severity of MR was assessed by RJA/LAA and subtraction method. No difference . . .s were noted between the assays measuring the severity of MR by χ2 analysis. The observed agreement between the assays was 81% for RJA/LAA vs RegV and was 73% for RJA/LAA vs RF, and the kappa statistic values for RJA/LAA vs RegV and for RJA/LAA vs RF were 0.63 (substantial agreement) and 0.50 (moderate agreement), respectively. Our results indicate that each quantification method was valuable to estimate the acuteness of the disease in ASHs with MR and all were in good accordance with the echocardiographic heart size and N-terminal Pro-Brain Natriuretic Peptide (NT-proBNP) measurements. Therefore, the each of these non-invasive methods may be functional to serially estimate the acuteness of MR in DMVD in order to monitor the progression of disease. Future studies have to evaluate, if these will be useful to anticipate the risk or time of decompensation in asymptomatic dogs. - Key words: Anatolian shepherd dog, Canine, Cardiac, Degenerative mitral valve disease, Quantification of mitral regurgitatio More less